Histological, biomechanical and radiological evaluation of bone repair with human platelet rich plasma in rabbit model

This study was carried out to evaluation the effect of human platelet rich plasma [hPRP] on the bone repair process in rabbit model which could be used in many procedures of orthopedic or maxillofacial bone and implant reconstructive surgery. This study is a prospective experimental study on animal model. A critical size defect [10 mm] was created in the radial diaphysis of 24 rabbit and then supplied with human PRP [treatment group] or the defect left empty [control group]. Radiographs of each forelimb was taken postoperatively on 1st day and at the 2nd, 4th, 6th and 8th weeks post injury to evaluate bone formation, union and remodeling of the defect. The operated radii were removed on 56th postoperative day and were evaluated for biomechanical properties and histopathological criteria. The results indicate that human PRP [as a xenogenic PRP] in treatment group significantly promote bone regeneration in critical size defects compared with control group [p<0.05]. This study showed that hPRP has a high regenerative capacity in critical size bone defects in rabbit model after 8 weeks

Evaluation of bone healing in canine tibial defects filled with cortical autograft, commercial-DBM, calf fetal DBM, omentum and omentum-calf fetal DBM

The present study was conducted to compare the effects of xenogenic bovine fetal demineralized bone matrix (DBM), commercial DBM, omentum, omentum-calf fetal DBM, cortical autograft and xenogenic cartilage powder on the healing of tibial defects in a dog model to determine the best material for bone healing. Seven male adult mongrel dogs, weighing 26.2 +/- 2.5 kg, were used in this study. Seven holes with a diameter of 4-mm were created and then filled with several biomaterials. Radiographs were taken postoperatively on day 1 and weeks 2, 4, 6, 8. The operated tibias were removed on the 56th postoperative day and histopathologically evaluated. On postoperative days 14, 42 and 56, the lesions of the control group were significantly inferior to those in the other group (p < 0.05). On the 28th postoperative day, the autograft group was significantly superior to the control and omentum groups (p < 0.05). Moreover, calf fetal DBM was significantly superior to the control group. There was no significant difference between the histopathological sections of all groups. Overall, the omentum and omentum-DBM groups were superior to the control group, but inferior to the autograft, commercial-DBM, calf fetal DBM and calf fetal cartilage groups.